The user can also draw upon the chakra of those bound to the tree, causing it to erupt from the ground and enter their own body. Over the course of years, humans connected to the tree in this manner are gradually drained of their life energy, personalities and defining features, reducing them to shells of their former selves and ultimately converting them into White Zetsu while at the same time, producing a new Chakra Fruit in the process. Victims of the Infinite Tsukuyomi are rendered immobile so long as they are under its effects, allowing them to easily be wrapped by the bark of the God Tree's roots using God: Nativity of a World of Trees. All those who fall under its gaze are trapped in the genjutsu, with the Rinnegan's design appearing in their eyes to represent its influence. Once cast, the penetrating light of the Infinite Tsukuyomi shines across the world, bright enough to dispel the night for as long as it is active.

The exact method of achieving this varies from user to user: in the anime Kaguya Ōtsutsuki uses Yomotsu Hirasaka to create a portal to a dimension containing a moon Obito Uchiha produces the Ten-Tails and transforms it into a construct of the God Tree, which has a Rinne Sharingan within the blooming flower at its top Madara Uchiha, per his Eye of the Moon Plan, flies into the sky and reflects his own Rinne Sharingan off of the moon. To cast a genjutsu of this scale, the user's Rinne Sharingan must be reflected off of the moon.

Infinite Tsukuyomi cast through the God Tree's blossoming flower.

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